GeneTel Laboratories LLC

www.genetel-lab.com; info@genetel-lab.com; 1-877-248-4316

 

Custom Chicken and Rabbit Antibody Production Services

GeneTel provides low cost, high quality peptide and chicken antibody production services.  Followings are the prices and schedules.

 

Antigen-Ready Package: Chicken (CA-0001) $400 ; Rabbit (RCA-0001) $550;  60 days

Immunization of 2 hens or rabbit

IgY isolation from eggs or antiserum from rabbits

ELISA testing for antibody response

Final product: >600mg IgY antibodies and 5mg pre-immune IgY or 100ml rabbit antiserum

Standard Package: Chicken (CA-0002) $800; Rabbit (RCA-0002) $950;  80 days

Antigen design

15-mer (+) peptide synthesis, 15mg > 90% purity

Mass Spec and HPLC trace

Peptide conjugation to KLH

Immunization of 2 hens or rabbits

IgY isolation from eggs or antiserum from rabbits

ELISA testing for antibody response

Final product: >600mg IgY antibodies and 5mg pre-immune IgY or 100ml rabbit antiserum

Affinity Purified Package: Chicken (CA-0003) $1,200; Rabbit  (RCA-0003) $1350;  85 days

Antigen design

15-mer (+) peptide synthesis, 15mg > 90% purity

Mass Spec and HPLC trace

Peptide conjugation to KLH

Immunization of 2 hens or rabbits

IgY isolation from eggs or antiserum from rabbits

Affinity purification with peptide column

ELISA testing for antibody response

Final product: 2-5mg affinity pure antibodies and 1mg pre-immune IgY or IgG.

Affinity Purified Package: Chicken (CA-0004) $1,900; Rabbit (RCA-0004) $2050;  90 days

Antigen design

Synthesis of 2 peptides, 15mg phospho- and 10mg non-phospho-peptide, 90+%

Mass Spec and HPLC trace

Peptide conjugation to KLH

Immunization of 2 hens or rabbits

IgY isolation from eggs or antiserum from rabbits

Double affinity purification to yield phospho specific antibody

ELISA testing for antibody response

Final product: about 2mg affinity pure antibodies and 1mg pre-immune IgY or IgG.

 

Mouse monoclonal antibody production: Customer provide antigen (MAB0001) $5000, about 6 months

Mouse monoclonal antibody production: Peptide antigen made in-house (MAB0001P) $5200, about 7 months

Please email to inquire more info (info@genetel-lab.com)

MORE DETAIL FOR CUSTOM CHICKEN ANTIBODY PRODUCTION (Item# CA-0001)

Customer provides ready-to-immunize antigen

Chicken set up per hen                                                                                                              $15

Immunization*: Intramuscular (3 times)                                                                                       $10 each

Subcutaneous                                                                                                                            $10

Termination                                                                                                                               $15   

IgY isolation from 10 eggs (provide >500mg crude IgY)**                                                           $75

Daily maintenance fee (60-70 day)***                                                                                        $1 per day

 

Estimated total cost per hen is about $200, per project with 2 hens: $400.  Customer provide ready-to-immunize antigen

 

(Freight-Federal Express Priority overnight, customer pays for the shipment)

* All injections included Freund’s adjuvant, other adjuvants are available at an additional cost; four injections are normally recommended and at least 2 hens are recommended.

** One pre-immune egg and about 20 immune eggs are normally collected from each hen.  The immune eggs will be collected 1 weeks after the second immunization.

***A total of about 60 days is normally recommended.  Extended period can be requested with additional cost of $1 per day.  Daily maintenance includes care, feeding and veterinary care if necessary.

NOTE:  We will not accept radioactive, toxic, or infectious antigens.

 

Please send inquiry for custom antibody production in other species to: info@genetel-lab.com

 

 

ANTIBODY PURIFICATION

 

1.         Antibody Purification (AP-1)- Total IgY (about 50mg/egg): IgY will be purified to about 90% purity and protein concentration will be determined. $20/egg for individual egg, $10/egg for pooled eggs. Total cost is about $360 (12 x $20 = $240 for individual eggs, 12 x $10 = $120 for pooled eggs) for 500-1000mg IgY. 

 

2.   Antibody Purification (AP-2): This service includes antibody purification using Protein-A affinity chromatography. Price: $ 65 for 5-10 mg IgG (human IgG, mouse IgG, rabbit IgG and bovine IgG), $85 for 10-30 mg, $105 for 30-50 mg, $125 for 50-100 mg, $650 per g and $500 per g for > 2 g, For goat IgG, add 15% more.

 

3.   Antibody Purification (AP-3): This service includes antibody purification using Protein-G affinity chromatography. Price: $ 85 for 10-20 mg IgG, $105 for 20-40 mg, $135 for 40-60 mg, $175 for 60-100 mg, $925 per g, $750 per g for >2 g.

 

4.   Antibody Purification (AP-4): This service includes antibody purification using Protein-L affinity chromatography. Price: $ 90 for 10-20 mg IgG, $105 for 20-40 mg, $135 for 40-60 mg, $165 for 60-100 mg, and $750 per g.

 

5.   Antibody Purification (AP-5): This service includes antibody purification using Protein-LA affinity chromatography. Price: $ 95 for 10-15 mg IgG, $115 for 20-40 mg, $135 for 40-60 mg, and $180 for 60-100 mg, and $950 per g.

 

6.  Affinity Purification (AP-6):  This service includes antibody purification using protein, peptide or other molecules made affinity chromatography.  Price:  $600 for yield from 2-10mg.   We need about 5-10mg free peptide, ideally peptide with a cystein at C or N terminal, or 5-10mg protein (or fusion).

 

 

SDS-PAGE of Antigens

 

Antigen Purification - about 3 mg antigen needed for four injections of 2-3 hens. Customer provides information on protein concentration and buffer conditions. The purity of target protein must be >30%. Cost: $160 per antigen.

 

 

CONJUGATION TO CUSTOMER SUPPLIED ANTIGEN

 

Conjugation of up to 10 mg of antigen to two conjugates (KLH or BSA) - Cost: $150

Conjugation of same antigen to an additional carrier – Cost: $100

 

 

ANTIBODY TITRATION BY ELISA

 

TITRATION ASSAY IN 96 WELL MICROPLATE 50 - 100ug antigen needed per plate for coating and protein concentration must be provided. Need to know buffer conditions

$200 for first microplate, $150 for each additional microplate

 

WESTERN BLOT ASSAY

 

This is a four-lane-assays. Need 100ug antigen, approximate molecular weight of protein of interest, protein concentration and buffer conditions. Cost: $200 for first four lanes, $100 for each additional four lanes.

 

 

BIOTINYLATION

 

We provide low background secondary Abs such as bovine anti-IgY biotin and anti-IgY HRP (see Conjugates Section). These Abs are suitable for ELISA, Western Blot and IHC/ICC.  However, we also provide biotinylation service.  Cost: $150/5mg. 

 

 

CUSTOM PEPTIDE SYNTHESIS

 

We help customers to find and design the best antigenic peptides as immunogens. In general, 10-20 aa peptides are selected as antigens. Cost: $30 per aa for 10-20mg MAP (multiple antigenic peptide with lysine branches) peptides.

 

WESTERN BLOT ANALYSIS PROTOCOL

Western Blot Analysis Protocol

 

1.                  After transferring proteins from SDS-PAGE to nitrocellulose membrane, block the membrane with blocking buffer (5% nonfat milk in TBS) for 1 hour at RT, or overnight in 4oC.

2.                  Dilute Abs accordingly in Abs dilution buffer (3% nonfat milk in TBST (TBS plus 0.1% Tween 20)) and add the Abs in each blot.

3.                  Shake at RT for at least 2 hour or keep in 4oC overnight.

4.                  Empty the solution and wash at least 3X10 minutes with TBST with shaking.

5.                  Dilute conjugate in 3% nonfat milk/TBST (Genetel Anti-IgY AP 1:3000) and incubate blots with the conjugate for 1 hour, shaking.

6.                  Empty the solution and wash at least 4X10 minutes each with TBST.

7.                  Wash 1X 10 minutes with TBS.

8.                  Lay the membrane in a flat container and do color development using AP substrate. 

 

Buffer

TBS:

NaCl                                        8g                    KCl                                          0.2g

Trizma Base                             1.21g               D.I. Water                                1L

Adjust pH to 8.6 with 1M HCl

Blocking Buffer: TBS + 5% Nonfat Dry Milk.

TBST:

1L TBS

1 ml Tween-20 (Polyoxyethylene-Sorbitan Monolaurate)

Abs Dilution Buffer: TBST + 3% Nonfat Dry Milk

 

Note: Due to the nature of IgY sticky property, you may need to increase Tween-20 in your TBST.